Type III ANOVA on main and interaction effects, and LM model summaries.
Prolactin
Chisq
Df
Pr(>Chisq)
(Intercept)
142.6249156
1
<0.0001
***
B12
0.0133596
1
0.9
ns
GDM_new
2.0584988
1
0.2
ns
Study
0.2833550
1
0.6
ns
GA_samp
153.0273355
1
<0.0001
***
B12:GDM_new
1.2736316
1
0.3
ns
GDM_new:Study
1.6999725
1
0.2
ns
B12:Study
0.2217536
1
0.6
ns
B12:GDM_new:Study
0.7321150
1
0.4
ns
HPL
Chisq
Df
Pr(>Chisq)
(Intercept)
27.4857978
1
<0.0001
***
B12
1.0580423
1
0.3
ns
GDM_new
6.0339287
1
0.01
*
Study
3.7853360
1
0.05
ns
GA_samp
1979.9428441
1
<0.0001
***
B12:GDM_new
3.8688047
1
0.05
ns
GDM_new:Study
7.9375646
1
0.005
**
B12:Study
0.0272714
1
0.9
ns
B12:GDM_new:Study
6.5247527
1
0.01
*
GH2
Chisq
Df
Pr(>Chisq)
(Intercept)
343.0252955
1
<0.0001
***
B12
6.3264944
1
0.01
*
GDM_new
3.1930263
1
0.07
ns
Study
0.7898003
1
0.4
ns
GA_samp
898.0854142
1
<0.0001
***
B12:GDM_new
1.2319261
1
0.3
ns
GDM_new:Study
2.8650730
1
0.09
ns
B12:Study
1.7622600
1
0.2
ns
B12:GDM_new:Study
0.9244163
1
0.3
ns
Insulin
model term
df1
df2
F.ratio
Chisq
p.value
1
B12
1
Inf
6.372
6.372
0.01
*
5
GDM_new
1
Inf
12.913
12.913
0.0003
***
7
Study
1
Inf
7.038
7.038
0.008
**
8
GA_samp
1
Inf
0.018
0.018
0.9
ns
2
B12:GDM_new
1
Inf
1.272
1.272
0.3
ns
4
B12:Study
1
Inf
1.629
1.629
0.2
ns
6
GDM_new:Study
1
Inf
0.909
0.909
0.3
ns
3
B12:GDM_new:Study
1
Inf
0.302
0.302
0.6
ns
HOMA.IR
model term
df1
df2
F.ratio
Chisq
p.value
1
B12
1
Inf
4.974
4.974
0.03
*
5
GDM_new
1
Inf
11.314
11.314
0.0008
***
7
Study
1
Inf
8.376
8.376
0.004
**
8
GA_samp
1
Inf
0.010
0.010
0.9
ns
2
B12:GDM_new
1
Inf
0.879
0.879
0.3
ns
4
B12:Study
1
Inf
1.091
1.091
0.3
ns
6
GDM_new:Study
1
Inf
0.677
0.677
0.4
ns
3
B12:GDM_new:Study
1
Inf
0.121
0.121
0.7
ns
GLUC3_mmol.L
model term
df1
df2
F.ratio
Chisq
p.value
1
B12
1
Inf
0.355
0.355
0.6
ns
5
GDM_new
1
Inf
0.786
0.786
0.4
ns
7
Study
1
Inf
8.298
8.298
0.004
**
8
GA_samp
1
Inf
0.579
0.579
0.4
ns
2
B12:GDM_new
1
Inf
0.044
0.044
0.8
ns
4
B12:Study
1
Inf
0.073
0.073
0.8
ns
6
GDM_new:Study
1
Inf
0.267
0.267
0.6
ns
3
B12:GDM_new:Study
1
Inf
0.010
0.010
0.9
ns
59.3.2 Model summary
Ratio of Geometric means (95% CI)
Prolactin
Var
Effect
P
(Intercept)
2.58 (2.16, 3.01)
<0.0001
B12
0.00 (-0.00, 0.00)
0.72
GDM_newGDM
-0.21 (-0.49, 0.08)
0.16
StudySTOP
-0.04 (-0.26, 0.19)
0.76
GA_samp
0.15 (0.13, 0.17)
<0.0001
B12:GDM_newGDM
0.00 (-0.00, 0.00)
0.36
GDM_newGDM:StudySTOP
0.16 (-0.06, 0.39)
0.16
B12:StudySTOP
0.00 (-0.00, 0.00)
0.84
HPL
Var
Effect
P
(Intercept)
0.59 (0.37, 0.81)
<0.0001
B12
0.00 (-0.00, 0.00)
0.30
GDM_newGDM
-0.36 (-0.65, -0.07)
0.01
StudySTOP
0.12 (-0.00, 0.24)
0.05
GA_samp
0.29 (0.28, 0.31)
<0.0001
B12:GDM_newGDM
0.00 (0.00, 0.00)
0.05
GDM_newGDM:StudySTOP
0.46 (0.14, 0.78)
0.005
B12:StudySTOP
0.00 (-0.00, 0.00)
0.87
B12:GDM_newGDM:StudySTOP
-0.00 (-0.00, -0.00)
0.01
GH2
Var
Effect
P
(Intercept)
-2.77 (-3.07, -2.48)
<0.0001
B12
0.00 (0.00, 0.00)
0.004
GDM_newGDM
-0.21 (-0.42, 0.00)
0.05
StudySTOP
0.10 (-0.06, 0.26)
0.24
GA_samp
0.26 (0.25, 0.28)
<0.0001
B12:GDM_newGDM
0.00 (-0.00, 0.00)
0.56
GDM_newGDM:StudySTOP
0.19 (0.03, 0.36)
0.02
B12:StudySTOP
-0.00 (-0.00, 0.00)
0.09
Insulin
Var
Effect
P
(Intercept)
2.87 (2.29, 3.46)
<0.0001
B12
-0.00 (-0.00, 0.00)
0.05
GDM_newGDM
0.97 (0.54, 1.40)
<0.0001
StudySTOP
-0.49 (-0.81, -0.16)
0.004
GA_samp
0.00 (-0.03, 0.04)
0.89
B12:GDM_newGDM
-0.00 (-0.00, 0.00)
0.27
GDM_newGDM:StudySTOP
-0.25 (-0.58, 0.08)
0.14
B12:StudySTOP
0.00 (-0.00, 0.00)
0.20
HOMA.IR
Var
Effect
P
(Intercept)
1.51 (0.89, 2.13)
<0.0001
B12
-0.00 (-0.00, 0.00)
0.07
GDM_newGDM
1.07 (0.59, 1.55)
<0.0001
StudySTOP
-0.64 (-0.99, -0.29)
0.0004
GA_samp
-0.00 (-0.04, 0.03)
0.92
B12:GDM_newGDM
-0.00 (-0.00, 0.00)
0.29
GDM_newGDM:StudySTOP
-0.31 (-0.67, 0.06)
0.10
B12:StudySTOP
0.00 (-0.00, 0.00)
0.23
GLUC3_mmol.L
Var
Effect
P
(Intercept)
1.76 (1.64, 1.87)
<0.0001
B12
-0.00 (-0.00, 0.00)
0.19
GDM_newGDM
0.11 (-0.04, 0.26)
0.16
StudySTOP
-0.16 (-0.22, -0.10)
<0.0001
GA_samp
-0.00 (-0.01, 0.00)
0.45
B12:GDM_newGDM
0.00 (-0.00, 0.00)
0.80
GDM_newGDM:StudySTOP
-0.08 (-0.20, 0.03)
0.15
B12:StudySTOP
0.00 (-0.00, 0.00)
0.52
59.3.3 Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.
Prolactin
Study
GDM_new
response
SE
df
lower.CL
upper.CL
SCOPE
Uncomplicated
95.12747
7.791555
108.87662
80.87298
111.8944
STOP
Uncomplicated
93.83962
7.020651
76.25041
80.84932
108.9171
SCOPE
GDM
86.24608
10.930774
483.07616
67.23380
110.6346
STOP
GDM
100.23681
8.826828
141.47889
84.22169
119.2973
HPL
Study
GDM_new
response
SE
df
lower.CL
upper.CL
SCOPE
Uncomplicated
86.68206
2.632632
213.4706
81.64505
92.02982
STOP
Uncomplicated
98.85500
2.509604
106.3658
94.00280
103.95767
SCOPE
GDM
82.65049
4.849031
610.8268
73.65582
92.74357
STOP
GDM
95.63783
3.401521
314.8558
89.17405
102.57015
GH2
Study
GDM_new
response
SE
df
lower.CL
upper.CL
SCOPE
Uncomplicated
2.304977
0.0803862
513.1083
2.152340
2.468440
STOP
Uncomplicated
2.219625
0.0577786
208.4185
2.108593
2.336504
SCOPE
GDM
1.970136
0.1533418
1081.8609
1.691104
2.295208
STOP
GDM
2.296205
0.0948462
488.7588
2.117210
2.490332
Insulin
Study
GDM_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
Uncomplicated
13.55675
0.9031283
Inf
11.89734
15.44761
STOP
Uncomplicated
10.35361
0.4308209
Inf
9.54273
11.23339
SCOPE
GDM
29.66964
4.3870159
Inf
22.20505
39.64357
STOP
GDM
17.64377
1.3367027
Inf
15.20911
20.46817
HOMA.IR
Study
GDM_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
Uncomplicated
3.289451
0.2333498
Inf
2.862465
3.780129
STOP
Uncomplicated
2.157247
0.0942625
Inf
1.980186
2.350140
SCOPE
GDM
7.863563
1.2846270
Inf
5.709042
10.831173
STOP
GDM
3.797184
0.3091261
Inf
3.237173
4.454073
GLUC3_mmol.L
Study
GDM_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
Uncomplicated
5.403450
0.0734390
Inf
5.261413
5.549322
STOP
Uncomplicated
4.707016
0.0423890
Inf
4.624664
4.790834
SCOPE
GDM
6.076550
0.3186762
Inf
5.482984
6.734373
STOP
GDM
4.865341
0.0638087
Inf
4.741872
4.992025
59.3.4 Comparisons
59.3.4.1 Within Groups
Effect for every 100 unit increase in B12 concentration (pM).
Hormone
Contrast
Study
GDM_new
Ratio of Geometric means (95% CI)
P-value
Prolactin
B12366 / B12266
SCOPE
Uncomplicated
1.010 (0.956, 1.067)
0.7
Prolactin
B12366 / B12266
STOP
Uncomplicated
1.017 (0.978, 1.058)
0.4
Prolactin
B12366 / B12266
SCOPE
GDM
1.045 (0.958, 1.140)
0.3
Prolactin
B12366 / B12266
STOP
GDM
1.052 (0.986, 1.123)
0.1
HPL
B12366 / B12266
SCOPE
Uncomplicated
1.016 (0.986, 1.047)
0.3
HPL
B12366 / B12266
STOP
Uncomplicated
1.019 (0.997, 1.041)
0.08
HPL
B12366 / B12266
SCOPE
GDM
1.122 (1.021, 1.233)
0.02
HPL
B12366 / B12266
STOP
GDM
0.977 (0.942, 1.015)
0.2
GH2
B12366 / B12266
SCOPE
Uncomplicated
1.060 (1.018, 1.103)
0.004
GH2
B12366 / B12266
STOP
Uncomplicated
1.016 (0.987, 1.045)
0.3
GH2
B12366 / B12266
SCOPE
GDM
1.077 (1.011, 1.148)
0.02
GH2
B12366 / B12266
STOP
GDM
1.032 (0.984, 1.083)
0.2
Insulin
B12366 / B12266
SCOPE
Uncomplicated
0.911 (0.830, 1.000)
0.05
Insulin
B12366 / B12266
STOP
Uncomplicated
0.976 (0.925, 1.029)
0.4
Insulin
B12366 / B12266
SCOPE
GDM
0.858 (0.751, 0.980)
0.02
Insulin
B12366 / B12266
STOP
GDM
0.919 (0.835, 1.011)
0.08
HOMA.IR
B12366 / B12266
SCOPE
Uncomplicated
0.910 (0.824, 1.006)
0.07
HOMA.IR
B12366 / B12266
STOP
Uncomplicated
0.975 (0.920, 1.034)
0.4
HOMA.IR
B12366 / B12266
SCOPE
GDM
0.856 (0.741, 0.987)
0.03
HOMA.IR
B12366 / B12266
STOP
GDM
0.917 (0.827, 1.016)
0.10
GLUC3_mmol.L
B12366 / B12266
SCOPE
Uncomplicated
0.989 (0.973, 1.005)
0.2
GLUC3_mmol.L
B12366 / B12266
STOP
Uncomplicated
0.996 (0.986, 1.005)
0.4
GLUC3_mmol.L
B12366 / B12266
SCOPE
GDM
0.992 (0.960, 1.026)
0.7
GLUC3_mmol.L
B12366 / B12266
STOP
GDM
0.999 (0.978, 1.020)
0.9
59.3.4.2 Between Groups
Below are comparisons of the differences in the change in B12 concentration (pM) between groups. I.e. whether the change in B12 concentration (pM) are different between groups.
Prolactin
contrast
ratio
SE
df
lower.CL
upper.CL
p.value
SCOPE/SCOPE (Overall)
1.013692
0.0682250
1341.524
0.8883123
1.156769
0.840
ns
STOP/SCOPE (Uncomplicated)
1.006823
0.0338813
1341.524
0.9425032
1.075532
0.840
ns
STOP/SCOPE (GDM)
1.006823
0.0338813
1341.524
0.9425032
1.075532
0.840
ns
GDM/Uncomplicated (SCOPE)
1.034630
0.0387836
1372.494
0.9612779
1.113578
0.364
ns
GDM/Uncomplicated (STOP)
1.034630
0.0387836
1372.494
0.9612779
1.113578
0.364
ns
HPL
contrast
ratio
SE
df
lower.CL
upper.CL
p.value
SCOPE/SCOPE (Overall)
0.8739563
0.0481123
664.0487
0.7844125
0.9737219
0.015
*
STOP/SCOPE (Uncomplicated)
1.0031428
0.0190608
1300.0835
0.9664378
1.0412418
0.869
ns
STOP/SCOPE (GDM)
0.8712183
0.0450833
600.1575
0.7870286
0.9644139
0.008
**
GDM/Uncomplicated (SCOPE)
1.1042956
0.0556984
563.4220
1.0001384
1.2193001
0.050
*
GDM/Uncomplicated (STOP)
0.9590684
0.0210968
1400.5208
0.9185638
1.0013591
0.058
ns
GH2
contrast
ratio
SE
df
lower.CL
upper.CL
p.value
SCOPE/SCOPE (Overall)
0.9187595
0.0451804
1392.880
0.8342711
1.011804
0.085
ns
STOP/SCOPE (Uncomplicated)
0.9585194
0.0235678
1392.880
0.9133844
1.005885
0.085
ns
STOP/SCOPE (GDM)
0.9585194
0.0235678
1392.880
0.9133844
1.005885
0.085
ns
GDM/Uncomplicated (SCOPE)
1.0164068
0.0280875
1431.273
0.9627764
1.073025
0.556
ns
GDM/Uncomplicated (STOP)
1.0164068
0.0280875
1431.273
0.9627764
1.073025
0.556
ns
Insulin
contrast
ratio
SE
df
asymp.LCL
asymp.UCL
p.value
SCOPE/SCOPE (Overall)
1.1469112
0.1224671
Inf
0.9303332
1.413908
0.199
ns
STOP/SCOPE (Uncomplicated)
1.0709394
0.0571774
Inf
0.9645378
1.189078
0.199
ns
STOP/SCOPE (GDM)
1.0709394
0.0571774
Inf
0.9645378
1.189078
0.199
ns
GDM/Uncomplicated (SCOPE)
0.9414962
0.0512245
Inf
0.8462658
1.047443
0.268
ns
GDM/Uncomplicated (STOP)
0.9414962
0.0512245
Inf
0.8462658
1.047443
0.268
ns
HOMA.IR
contrast
ratio
SE
df
asymp.LCL
asymp.UCL
p.value
SCOPE/SCOPE (Overall)
1.1477984
0.1316612
Inf
0.9166981
1.437159
0.229
ns
STOP/SCOPE (Uncomplicated)
1.0713535
0.0614462
Inf
0.9574435
1.198816
0.229
ns
STOP/SCOPE (GDM)
1.0713535
0.0614462
Inf
0.9574435
1.198816
0.229
ns
GDM/Uncomplicated (SCOPE)
0.9398504
0.0551365
Inf
0.8377662
1.054374
0.290
ns
GDM/Uncomplicated (STOP)
0.9398504
0.0551365
Inf
0.8377662
1.054374
0.290
ns
GLUC3_mmol.L
contrast
ratio
SE
df
asymp.LCL
asymp.UCL
p.value
SCOPE/SCOPE (Overall)
1.013107
0.0205779
Inf
0.9735669
1.054252
0.521
ns
STOP/SCOPE (Uncomplicated)
1.006532
0.0102222
Inf
0.9866950
1.026768
0.521
ns
STOP/SCOPE (GDM)
1.006532
0.0102222
Inf
0.9866950
1.026768
0.521
ns
GDM/Uncomplicated (SCOPE)
1.003341
0.0130778
Inf
0.9780336
1.029303
0.798
ns
GDM/Uncomplicated (STOP)
1.003341
0.0130778
Inf
0.9780336
1.029303
0.798
ns
59.3.5 Estimated means (50 unit increase)
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
Results are averaged over the levels of B12 concentration (pM) and may be misleading when interaction term is involved.
Prolactin
Study
GDM_new
response
SE
df
lower.CL
upper.CL
SCOPE
Uncomplicated
94.89006
7.689011
104.38962
80.80501
111.4303
STOP
Uncomplicated
93.44643
6.980985
75.81143
80.52703
108.4386
SCOPE
GDM
85.30174
10.708996
469.99571
66.65305
109.1681
STOP
GDM
98.97089
8.685380
139.61786
83.20617
117.7225
HPL
Study
GDM_new
response
SE
df
lower.CL
upper.CL
SCOPE
Uncomplicated
86.33838
2.557415
194.8997
81.43912
91.53238
STOP
Uncomplicated
98.38586
2.489064
104.9517
93.57222
103.44712
SCOPE
GDM
80.30615
4.473273
621.1605
71.98499
89.58919
STOP
GDM
96.18367
3.397694
307.3219
89.72505
103.10720
GH2
Study
GDM_new
response
SE
df
lower.CL
upper.CL
SCOPE
Uncomplicated
2.271758
0.0764773
464.7386
2.126337
2.427125
STOP
Uncomplicated
2.210930
0.0572560
204.9840
2.100877
2.326747
SCOPE
GDM
1.933858
0.1486430
1066.7735
1.663122
2.248668
STOP
GDM
2.277922
0.0931829
478.3484
2.101989
2.468581
Insulin
Study
GDM_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
Uncomplicated
13.87606
0.8774446
Inf
12.258601
15.70693
STOP
Uncomplicated
10.41744
0.4270716
Inf
9.613143
11.28903
SCOPE
GDM
30.82962
4.5440752
Inf
23.094412
41.15565
STOP
GDM
18.02213
1.3612571
Inf
15.542210
20.89775
HOMA.IR
Study
GDM_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
Uncomplicated
3.367533
0.2268849
Inf
2.950956
3.842916
STOP
Uncomplicated
2.170726
0.0935500
Inf
1.994902
2.362047
SCOPE
GDM
8.176041
1.3385987
Inf
5.931746
11.269472
STOP
GDM
3.880629
0.3154237
Inf
3.309140
4.550814
GLUC3_mmol.L
Study
GDM_new
response
SE
df
asymp.LCL
asymp.UCL
SCOPE
Uncomplicated
5.418350
0.0700905
Inf
5.282702
5.557481
STOP
Uncomplicated
4.712319
0.0423122
Inf
4.630114
4.795983
SCOPE
GDM
6.088227
0.3250731
Inf
5.483300
6.759891
STOP
GDM
4.866762
0.0636995
Inf
4.743501
4.993226
59.3.6 Comparisons (50 unit increase)
59.3.6.1 Within Groups
Effect for every 50 unit increase in B12 concentration (pM).
Hormone
Contrast
Study
GDM_new
Ratio of Geometric means (95% CI)
P-value
Prolactin
B12316 / B12266
SCOPE
Uncomplicated
1.005 (0.978, 1.033)
0.7
Prolactin
B12316 / B12266
STOP
Uncomplicated
1.008 (0.989, 1.028)
0.4
Prolactin
B12316 / B12266
SCOPE
GDM
1.022 (0.979, 1.068)
0.3
Prolactin
B12316 / B12266
STOP
GDM
1.026 (0.993, 1.060)
0.1
HPL
B12316 / B12266
SCOPE
Uncomplicated
1.008 (0.993, 1.023)
0.3
HPL
B12316 / B12266
STOP
Uncomplicated
1.010 (0.999, 1.021)
0.08
HPL
B12316 / B12266
SCOPE
GDM
1.059 (1.010, 1.110)
0.02
HPL
B12316 / B12266
STOP
GDM
0.989 (0.970, 1.007)
0.2
GH2
B12316 / B12266
SCOPE
Uncomplicated
1.029 (1.009, 1.050)
0.004
GH2
B12316 / B12266
STOP
Uncomplicated
1.008 (0.994, 1.022)
0.3
GH2
B12316 / B12266
SCOPE
GDM
1.038 (1.005, 1.071)
0.02
GH2
B12316 / B12266
STOP
GDM
1.016 (0.992, 1.041)
0.2
Insulin
B12316 / B12266
SCOPE
Uncomplicated
0.955 (0.911, 1.000)
0.05
Insulin
B12316 / B12266
STOP
Uncomplicated
0.988 (0.962, 1.015)
0.4
Insulin
B12316 / B12266
SCOPE
GDM
0.926 (0.867, 0.990)
0.02
Insulin
B12316 / B12266
STOP
GDM
0.958 (0.914, 1.005)
0.08
HOMA.IR
B12316 / B12266
SCOPE
Uncomplicated
0.954 (0.908, 1.003)
0.07
HOMA.IR
B12316 / B12266
STOP
Uncomplicated
0.988 (0.959, 1.017)
0.4
HOMA.IR
B12316 / B12266
SCOPE
GDM
0.925 (0.861, 0.994)
0.03
HOMA.IR
B12316 / B12266
STOP
GDM
0.957 (0.910, 1.008)
0.10
GLUC3_mmol.L
B12316 / B12266
SCOPE
Uncomplicated
0.995 (0.986, 1.003)
0.2
GLUC3_mmol.L
B12316 / B12266
STOP
Uncomplicated
0.998 (0.993, 1.003)
0.4
GLUC3_mmol.L
B12316 / B12266
SCOPE
GDM
0.996 (0.980, 1.013)
0.7
GLUC3_mmol.L
B12316 / B12266
STOP
GDM
0.999 (0.989, 1.010)
0.9
59.3.6.2 Between Groups
Below are comparisons of the differences in the change in B12 concentration (pM) between groups. I.e. whether the change in B12 concentration (pM) are different between groups.